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Biodegradable Mg-6Zn Alloy Down-Regulation the NF-B Signaling Pathway of Intestinal Epithelial Cells
发表时间:2016-07-01 阅读次数:5323次

Journal of Biomaterials and Tissue Engineering
Vol. 6, 1–7, 2016
Biodegradable Mg-6Zn Alloy Down-Regulation the NF-B
Signaling Pathway of Intestinal Epithelial Cells
Yigang Chen1†, Jiazeng Xia1†, Guidi Fan1, Kaiyuan Deng1, Hao Wang1, Xiaonong Zhang2,
Shaoxiang Zhang3, Changli Zhao2 , and Jun Yan 4
1Department of General Surgery, Nanjing Medical University Affiliated Wuxi Second Hospital, Jiangsu 214002, P. R. China
2State Key Laboratory of Metal Matrix Composites, School of Materials Science and Engineering,
Shanghai Jiao Tong University, Shanghai 200240, P. R. China
3Suzhou Origin Medical Technology Co. Ltd., Changshu Economic and Technology Development Zone, Jiangsu 215513, P. R. China
4Department of General Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai 200233, P. R. China

Abstract

Nuclear factor kappa B (NF-B) signaling pathway plays an important role in the processes of
immunity, inflammation, and cancer. As studies of magnesium (Mg)-zinc (Zn) alloy have increased,
it becomes necessary to investigate effects of Mg-6Zn alloy on NF-B signaling pathway. The Mg-
6Zn alloy was investigated in both in vitro and in vivo experiments. The mRNA expression of P65,
P50 and IB, and the protein expressions of pIB were tested using real time-PCR and western
blot analysis. In the in vivo experiments, pins of Mg-6Zn alloy were implanted into the intestinal
wall of SD rats. After 1 week, H and E staining and immunohistochemical analysis of NF-B, was
performed. Results of this study showed the expression of IB, pIB, P50 and P65 did not
significantly fluctuate in the 20% and 40% concentrations of Mg-6Zn alloy extracts. In the 80% and
100% extracts, the mRNA expression of IB was higher than that in the 0% extract. The mRNA
expression of P50 and P65 were significantly lower in the 60%, 80% and 100% extracts than those
in the 0% extract. The protein level of pIB was lower in the 60%, 80% and 100% extracts. In the
in vivo experiments, there were fewer inflammation and lower levels of expression of NF-B in the
Mg-6Zn alloy group than those in the control group. The NF-B signal pathway of IEC-6 cells is
sensitive to the concentration of the Mg-6Zn extracts. High concentrations extracts (60%, 80% and
100%) partly inhibit the NF-B signal pathway. The IB is the main acting sit of Mg-6Zn extracts.
The High concentrations Mg-6Zn extracts increase levels of IB and decrease IB degradation.
Keywords: Magnesium Alloy, NF-B Signaling Pathway, Intestinal Epithelial Cells.

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